mirabilis is considered as the causative pathogen in hospital cross-infection associated with urinary catheter ( O’Hara et al., 2000 Hola et al., 2012). Proteus mirabilis is a gram-negative bacterium with flagella around the body, which is widely found in water, soil, and human intestinal environments. mirabilis isolated from the food poisoning incident. In addition, type IV secretion system (T4SS) was preliminarily confirmed to play an important role in the pathogenesis of diarrheal P. mirabilis C02011 shows more gastrointestinal pathogenicity than the other strains isolated from a healthy individual. On the basis of these results, we firstly validated that the novel strain of P. C02011 strain shows more virulence than B02005 strain in terms of the following indicators: (1) feces water content and fecal character (2) colon length of mice (3) histological examination on mouse intestine tissues (4) ELISA for detecting TNF-α level in the colon and (5) WB and immunohistochemistry (IHC) for detecting occludin protein expression in the colon. In vivo, BALB/c mice were infected with P. Meanwhile, the adhesive abilities of different strains were rated as follows: P. After that, Giemsa staining and TEM were used for observing the infection process of C02011 strain. In vitro, Caco-2 cells were infected with P. According to the results, C02011 strain exhibited almost identical characteristics with B02005 strain in bacterial appearance and proliferation. mirabilis American Type Culture Collection (ATCC) 29906 strain both in vitro and in vivo. mirabilis B02005 strain (isolated from healthy people) and P. In order to determine its gastrointestinal pathogenicity, experiments were performed to compare P. mirabilis C02011 isolated from patients’ specimens in a food poisoning in China. In our study, we described a novel strain of P.
mirabilis were found to be associated with food poisoning outbreaks, with the pathogenic mechanism still unclear. Proteus mirabilis is commonly considered to be an opportunistic pathogen causing urinary tract infections (UTIs) in humans. 2Shenzhen Major Infectious Disease Control Key Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
1Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Diseases, School of Public Health, Southern Medical University, Guangzhou, China.Zelong Gong 1†, Xiaolu Shi 2*†, Fang Bai 2, Xiaolong He 1, Hanyun Zhang 1, Yubin Li 1, Yu Wan 1, Yiman Lin 2, Yaqun Qiu 2, Qiongcheng Chen 2, Qinghua Hu 2* and Hong Cao 1*